probe hybridization buffer Search Results


probe-hybridization buffer  (Molecular Instruments)
90
Molecular Instruments probe-hybridization buffer
Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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centromeric enumeration probe hybridization buffer  (Abbott Laboratories)
90
Abbott Laboratories centromeric enumeration probe hybridization buffer
Centromeric Enumeration Probe Hybridization Buffer, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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centromeric enumeration probe hybridization buffer - by Bioz Stars, 2026-03
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hcr probe hybridization buffer  (Molecular Instruments)
90
Molecular Instruments hcr probe hybridization buffer
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
Hcr Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcr probe hybridization buffer/product/Molecular Instruments
Average 90 stars, based on 1 article reviews
hcr probe hybridization buffer - by Bioz Stars, 2026-03
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probe hybridization buffer phb  (Molecular Instruments)
90
Molecular Instruments probe hybridization buffer phb
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
Probe Hybridization Buffer Phb, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/probe hybridization buffer phb/product/Molecular Instruments
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mirna5100 probe in lna hybridization buffer mixture  (Qiagen)
90
Qiagen mirna5100 probe in lna hybridization buffer mixture
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
Mirna5100 Probe In Lna Hybridization Buffer Mixture, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scramble probe in lna hybridization buffer mixture  (Qiagen)
90
Qiagen scramble probe in lna hybridization buffer mixture
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
Scramble Probe In Lna Hybridization Buffer Mixture, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna probes (600 ng/ml) in hybridization buffer  (Blackwell Science Ltd)
90
Blackwell Science Ltd rna probes (600 ng/ml) in hybridization buffer
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
Rna Probes (600 Ng/Ml) In Hybridization Buffer, supplied by Blackwell Science Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna probes (600 ng/ml) in hybridization buffer/product/Blackwell Science Ltd
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fish probes diluted hybridization buffer  (Empire Genomics)
90
Empire Genomics fish probes diluted hybridization buffer
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
Fish Probes Diluted Hybridization Buffer, supplied by Empire Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oligonucleotide probes complementary to rno-mir-155-5p  (Servicebio Inc)
90
Servicebio Inc oligonucleotide probes complementary to rno-mir-155-5p
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
Oligonucleotide Probes Complementary To Rno Mir 155 5p, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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probe mixture (probe/hybridization buffer/purified h 2 o=1:7:2)  (Beijing GP Medical Technologies)
90
Beijing GP Medical Technologies probe mixture (probe/hybridization buffer/purified h 2 o=1:7:2)
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
Probe Mixture (Probe/Hybridization Buffer/Purified H 2 O=1:7:2), supplied by Beijing GP Medical Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hybridization buffer 4 nmol/l probe mixture  (Molecular Instruments)
90
Molecular Instruments hybridization buffer 4 nmol/l probe mixture
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
Hybridization Buffer 4 Nmol/L Probe Mixture, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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30% lmw probe hybridization buffer  (Molecular Instruments)
90
Molecular Instruments 30% lmw probe hybridization buffer
A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.
30% Lmw Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: hybridization chain reaction (HCR) in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.

Journal: Nature Communications

Article Title: Cell-type-specific mRNA transcription and degradation kinetics in zebrafish embryogenesis from metabolically labeled single-cell RNA-seq

doi: 10.1038/s41467-024-47290-9

Figure Lengend Snippet: A A UMAP projection of 8226 single cells with five cell lineages (colors as indicated). Each cell is colored by its lineage assignments. B Histograms (y-axis; number of genes) of trajectory-specific maternal (red, top) and zygotic (blue, bottom) significantly differentially regulated genes, per trajectory (x-axis, color-coded as in ( A )). Each set of trajectory-specific genes is divided into up-regulated (right) and down-regulated (left) genes. Some genes are differentially regulated in more than one trajectory. C – E Trajectory-specific model fits (solid lines) to interpolated zygotic (right) trajectory (light blue dots) or non-trajectory (dark blue dots) and maternal (left) trajectory (light red dots) or non-trajectory (dark red dots) expression levels (y-axis, log 2 scale) across 11 pseudotime bins (x-axis) for genes with trajectory specific regulation. Expression levels were interpolated separately for cells that are assigned to a trajectory or those not assigned to it across 11 pseudotime bins. Gene name and trajectory are indicated on top. Gray lines represent fits that match both trajectory and non-trajectory data, and therefore retain the null hypothesis of similar regulation within and outside a trajectory. F Left: hybridization chain reaction (HCR) in situs against dendra mRNA with “neutral“ UTRs, mOxBFP mRNA with “neutral UTRs”, and krt8 that marks enveloping layer (EVL) cells. Right: diagrams of injected reporter mRNAs. G Quantification of ratio of mOxBFP to dendra HCR fluorescence in the enveloping layer (EVL) and deep layer (rest of the blastoderm) in “neutral” UTR reporter injections from n = 6 independent embryos. Box plots show median (line), 1st and 3rd quartiles (hinges), and 1.5 inter-quartile ranges (whiskers). Significance was calculated using a two-sided Student’s t test. Each sample was measured twice in the two different regions. H , I Similar to ( F , G ), except using mOxBFP mRNA with epcam UTRs in n = 12 independent embryos.

Article Snippet: Embryos were then pre-hybridized in HCR probe hybridization buffer (Molecular Instruments) for 2 h at 37 °C with shaking at 300 rpm in a ThermoMixer C. To prepare probe working solution, 1 μL of each 1 μM HCR probe was diluted in 500 μL of probe hybridization buffer at 37 °C.

Techniques: Expressing, Hybridization, Injection, Fluorescence